Methionine Adenosyltransferase (MAT) Activity BioAssay(TM) Kit

Produktübersicht

• Artikelname: Methionine Adenosyltransferase (MAT) Activity BioAssay(TM) Kit
• Artikelnummer: USB-353211
• Hersteller Artikelnummer: 353211
• Alternativnummer: USB-353211-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Methionine Adenosyltransferase (MAT) activity assay kit Sample Type: Yeast and other expression system, tissue Applications: Levels of MAT1A/1B, MAT2A gene expressions. Extent of turning methionine into active methionine, i.e., SAM, related to methylation capability. Test Principle: This kit is designed to calculate MAT activity through measuring the concentration of the main product SAM. The chemical reaction and the competition cELISA are carried out simultaneously, so that the SAM synthesized from the chemical reaction competes with antigens coated on the microtiter plate for the horseradish peroxidase conjugated anti-SAM antibody the minute it is generated. The SAM quantified based on the amount of the HRP-anti-SAM antibodies that was made not to bind to the microtiter plate. Kit Components: 353211A: Microtiter Plate, 1x96 wells 353211B: HRP Diluent, 1x9ml *353211C: HRP Antibody, 1x15ul 353211D: MAT Substrate, 1x1ml 353211E: Sample Diluent, 1x6ml 353211F: MAT Positive Control, 1x40ul 353211G: TMB Substrate, 1x11ml 353211H: Stop Solution, 1x6ml 353211J: SAM Standard, 0nM, 1x200ul 353211K: SAM Standard, 15nM, 1x200ul 353211L: SAM Standard, 30nM, 1x200ul 353211M: SAM Standard, 120nM, 1x200ul 353211N: SAM Standard, 240nM, 1x200ul 353211P: SAM Standard, 480nM, 1x200ul 353211Q: SAM Standard, 960nM, 1x200ul 353211R: SAM Quality Control, 160-240nM, 1x200ul 353211S: Wash Solution, 20X, 1x20ml Storage and Stability: Store *353211C at -20C. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.