4-Hydroxynonenal (4-HNE) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: 4-Hydroxynonenal (4-HNE) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-353545
• Hersteller Artikelnummer: 353545
• Alternativnummer: USB-353545-48, USB-353545-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The 4-Hydroxynonenal (4-HNE) BioAssay(TM) ELISA Kit is a quantitative competitive assay for the detection of 4-HNE in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples. Detection Range: 31.25-2000pg/ml Sensitivity: 18.75pg/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Assay Principle: This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with 4-HNE. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to 4-HNE. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 4-HNE, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 4-HNE in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *353545A: Microtiter Strips, 1x48 wells or 1x96 wells *353545B: Standard, 1x1 vial or 2x1 vials 353545C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 353545D: Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul 353545E: Antibody Dilution Buffer, 1x5ml or 1x10ml 353545F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul 353545G: SABC Dilution Buffer, 1x5ml or 1x10ml 353545H: TMB Substrate, 1x5ml or 1x10ml 353545J: Stop Solution, 1x5ml or 1x10ml 353545K: Wash Buffer, 25X, 1x15ml or 1x30ml Storage and Stability: Store unopened *353545A at 4C, store at -20C once opened. Store unopened *353545B at 4C, once reconstituted, store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards and samples to wells. 2. Add 50ul standard or sample to each well. 3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.