Amyloid Beta 42 (AB42) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: Amyloid Beta 42 (AB42) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-353586
• Hersteller Artikelnummer: 353586
• Alternativnummer: USB-353586-48,USB-353586-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The Amyloid beta 42 (AB42) BioAssay(TM) ELISA Kit (Human) is a quantitative sandwich assay for the detection of Amyloid beta 42 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples. Detection Range: 15.625-1000pg/ml Sensitivity: <9.375pg/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Kit Components: *353586A: Microtiter Strips, 8x12 wells. *353586B: Standard, 2x1 vial 353586C: Sample/Standard Dilution Buffer, 1x20ml 353586D: Antibody-Biotin Concentrate, 1x120ul 353586E: Antibody Dilution Buffer, 1x10ml *353586F: Streptavidin-HRP Conjugate (SABC), 1x120ul 353586G: SABC Dilution Buffer, 1x10ml 353586H: TMB Substrate, 1x10ml *353586J: Stop Solution, 1x10ml 353586K: Wash Buffer, 25X, 1x30ml Storage and Stability: Store unopened *353586A at 4C, store at -20C once opened. Store unopened *353586B at 4C, once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store *353586F and *353586J at 4C in the dark. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid beta 42. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to Amyloid beta 42. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amyloid beta 42, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm. The concentration of Amyloid beta 42 in the sample is then determined by comparing the O.D. of the sample to the standard curve. Assay Summary: 1. Wash plate 2 times before adding standards, samples and control (zero) to wells. 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37C. Aspirate and wash 2 times. 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.