Creatine Kinase MB Isoenzyme (CK-MB) BioAssay(TM) ELISA Kit (Rat)

Produktübersicht

• Artikelname: Creatine Kinase MB Isoenzyme (CK-MB) BioAssay(TM) ELISA Kit (Rat)
• Artikelnummer: USB-354798
• Hersteller Artikelnummer: 354798
• Alternativnummer: USB-354798-48, USB-354798-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The Creatine Kinase MB Isoenzyme (CK-MB) BioAssay(TM) ELISA Kit is a quantitative sandwich assay for the detection of CK-MB in rat serum, plasma, tissue homogenates and other biological fluids. Detection Range: 31.25-2000pg/ml Sensitivity: 18.75pg/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CK-MB. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to CK-MB. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CK-MB, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of CK-MB in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *354798A: Microtiter Strips, 1x48 wells or 1x96 wells *354798B: Standard, 1x1vial or 2x1vials 354798C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 354798D: Antibody-Biotin Concentrate, 1x60ul or 1x120ul 354798E: Antibody Dilution Buffer, 1x5ml or 1x10ml 354798F: Streptavidin-HRP Conjugate (SABC), 1x60ul or 1x120ul 354798G: SABC Dilution Buffer, 1x5ml or 1x10ml 354798H: TMB Substrate, 1x5ml or 1x10ml 354798J: Stop Solution, 1x5ml or 1120ml 354798K: Wash Buffer, 25X, 1x15ml or 1x30ml Storage and Stability: Store unopened *354798A at 4C, store at -20C once opened. Store unopened *354798B at 4C, once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards, samples and control (zero) to wells. 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37C. Aspirate and wash 2 times. 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.