High mobility group protein B1 (HMGB1) BioAssay(TM) ELISA Kit (Mouse)

Produktübersicht

• Artikelname: High mobility group protein B1 (HMGB1) BioAssay(TM) ELISA Kit (Mouse)
• Artikelnummer: USB-356299
• Hersteller Artikelnummer: 356299
• Alternativnummer: USB-356299-48, USB-356299-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The High mobility group protein B1 (HMGB1) BioAssay(TM) ELISA Kit is a quantitative sandwich assay for the detection of Hmgb1 in mouse serum, plasma, tissue homogenates and other biological fluids. Detection Range: 15.625-1000pg/ml Sensitivity: 9.375pg/ml Precision: Intra-assay CV: <8% Inter-assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMGB1. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to HMGB1. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HMGB1, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of HMGB1 in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *356299A: Microtiter Strips, 1x48 wells or 1x96 wells *356299B: Standard, 1x1vial or 2x1 vial 356299C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 356299D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul 356299E: Antibody Dilution Buffer, 1x5ml or 1x10ml 356299F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul 356299G: SABC Dilution Buffer, 1x5ml or 1x10ml 356299H: TMB Substrate, 1x5ml or 1x10ml 356299J: Stop Solution, 1x5ml or 1x10ml 356299K: Wash Buffer, 25X, 1x15ml or 1x30ml Storage and Stability: Store unopened *356299A at 4C, store at -20C once opened. Store unopened *356299B at 4C, once reconstituted, store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards and samples to wells! 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37C. Aspirate and wash 2 times. 3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results