ADAMTS12 (A Disintegrin And Metalloproteinase With Thrombospondin 12), Rabbit
Artikelnummer:
USB-362000
Hersteller Artikelnummer:
362000
Alternativnummer:
USB-362000-200
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Gln823-Pro997 of human ADAMTS12, expressed in E. coli.
ADAMTS12 possesses all the domains characteristic of ADAMTSs, including signal sequence, propeptide, metalloproteinase-like, disintegrin-like, and cysteine-rich domains, as well as a series of 8 uniquely organized thrombospondin I (THBS1) repeats. Expression was upregulated by transforming growth factor-beta (TGFB) in fetal fibroblasts. RT-PCR analysis detected wide expression of ADAMTS12 in gastric carcinomas but not in adjacent normal tissues. ADAMTS12 is expressed as proteins of 83 and 120kD, considerably lower masses than the 17kD protein expressed in vitro. ADAMTS12 is extensively processed at its N-terminal end, after the cysteine-rich domain and around the first THBS1 domain, by a metalloprotease in cells. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGHHHHHHSG SEF- QQMYFWQY GHWTECSVTC GTGIRRQTAH CIKKGRGMVK ATFCDPETQP NGRQKKCHEK ACPPRWWAGE WEACSATCGP HGEKKRTVLC IQTMVSDEQA LPPTDCQHLL KPKTLLSCNR DILCPSDWTV GNWSECSVSC GGGVRIRSVT CAKNHDEPCD VTRKPNSRAL CGLQQCP Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
