MIP1b (Macrophage Inflammatory Protein 1 Beta, CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b), Rabbit
Artikelnummer:
USB-364155
Hersteller Artikelnummer:
364155
Alternativnummer:
USB-364155-200
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Met1-Asn92 of Simian MIP1b, expressed in E. coli.
The two variants of the Macrophage Inflammatory Protein 1, designated MIP-1alpha and MIP-1Beta, belong to the Beta or CC chemokine subfamily. The two chemokines were originally purified from lipopolysaccharidetreated murine monocytic cell lines, hence their designation as Macrophage Inflammatory Protein. MIP-1alpha and MIP-1Beta are produced by stimulated leukocytes as well as other tissue cells and tumor cells. At least seven variants of rat MIP-1Beta, corresponding to the products of independantly cloned cDNA, including Act-2, G-26, HC-21, HIMAP, H400, pAT 744 and MAD-5, have been described. At the protein levels, mature rat MIP-1Beta has a length of 69aa and shows a homology of 70% with MIP-1alpha. MIP-1Beta is a natural ligand for the chemokine receptors CCR3, CCR5 and CCR8. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
