POSTN (Periostin, OSF-2, PDLPOSTN, PN, Osteoblast Specific Factor 2), Rabbit
Artikelnummer:
USB-364718
Hersteller Artikelnummer:
364718
Alternativnummer:
USB-364718-200
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Arg659-Gln810 of rat POSTN, fused to N-terminal His-Tag expressed in E. coli.
By screening human placenta and osteosarcoma cDNA libraries with mouse Postn as probe, they cloned 2 variants of human POSTN. One variant encodes a deduced 779aa protein with an apparent molecular mass of 87.0kD, and the other encodes a deduced 836aa protein with an apparent molecular mass of 93.3kD. POSTN contains a typical signal sequence, followed by a cysteine-rich domain, a 4-fold repeat structure of about 150aa, and a C-terminal domain. Mouse and human POSTN share 89.2% amino acid identity overall and 90.1% identity in their mature forms. Northern blot analysis of a mouse osteoblastic cell line detected a 3.4-kb Postn transcript. RNA dot blot analysis detected expression of Postn in primary mouse osteoblasts and in lung, but not in any other tissues examined. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
