Recombinant protein corresponding to Met1-Ala312 of human PSAT1, expressed in E. coli.
PSAT catalyzes the second step in the pathway, conversion of 3-phosphohydroxypyruvate into 3-phosphoserine. The full-length PSAT-beta transcript encodes a deduced 370aa protein with a calculated molecular mass of 40kD. PSAT-alpha lacks exon 8 and encodes a deduced 324aa protein with a calculated molecular mass of 35.2kD. Compared with PSAT-beta, PSAT-alpha lacks 46aa. Both proteins contain a conserved binding domain for the cofactor pyridoxal 5-prime-phosphate (vitamin B6). PSAT-beta shares 92.4% aa similarity with its mouse homolog. PSAT-beta orthologs were present in all species examined, including plants, insects, and bacteria. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDAPRQVVNF GPGPAKLPHS VLLEIQKELL DYKGVGISVL EMSHRSSDFA KIINNTENLV RELLAVPDNY KVIFLQGGGC GQFSAVPLNL IGLKAGRCAD YVVTGAWSAK AAEEAKKFGT INIVHPKLGS YTKIPDPSTW NLNPDASYVY YCANETVHGV EFDFIPDVKG AVLVCDMSSN FLSKPVDVSK FGVIFAGAQK NVGSAGVTVV IVRDDLLGFA LRECPSVLEY KVQAGNSSLY NTPPCFSIYV MGLVLEWIKN NGGAAAMEKL SSIKSQTIYE IIDNSQGFYV SVGGIRASLY NAVTIEDVQK LA Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
