Recombinant protein corresponding to Met1-His155 of human RFK expressed in E. coli.
Riboflavin kinase (RFK, EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. The reference genome sequence contains a polymorphism that changes the upstream ATG found in most transcripts to ATT, hence the next downstream AUG is annotated. This polymorphism is represented in the Reference SNP, rs2490582. The reference genome polymorphism results in a protein that is 7 aa shorter at the N-terminus, and is supported by homologous proteins. It is also possible that leaky scanning by ribosomes would permit translation initiation from the downstream AUG in alleles containing the upstream AUG. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
