Recombinant protein corresponding to Gly21-Lys89 of rat SDF1, expressed in E. coli.
SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12).SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene. SDF-1beta is synthesized as a 93aa precursor that contains a 21aa signal sequence and a 72aa mature region. The mature molecule exhibits a typical three antiparallel beta-strand chemokine-like fold. There are no potential N-linked glycosylation sites. N-terminal amino acid s 1-8 form a receptor binding site, while aas 1 and 2 (Lys-Pro) are involved in receptor activation. The C-terminus is likely associated with heparin binding. SDF-1beta circulates and undergoes proteolytic processing. SDF-1beta are reported to be a monomers at neutral pH and physiologic ionic strength. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
