Recombinant protein corresponding to Pro2-Arg159 of human SNRPC, expressed in E. coli.
The C polypeptide is one of the specific protein constituents of the U1 snRNP (small nuclear ribonucleoprotein) particle. See SNRPA. Patients with connective tissue diseases often produce autoantibodies against snRNP particles. By screening human expression libraries with anti-RNP sera, both Yamamoto et al. (1988) and Sillekens et al. (1988) isolated cDNAs encoding SNRPC. Using Northern blot hybridization, Yamamoto et al. (1988) determined that SNRPC is expressed as an approximately 0.8-kb mRNA. Antibodies against SNRPC stained the nuclei of HeLa cells in a speckled pattern. Sillekens et al. (1988) reported that the predicted 159aa SNRPC protein has an apparent molecular mass of 22kD by SDS-PAGE. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PKFYCDYCD TYLTHDSPSV RKTHCSGRKH KENVKDYYQK WMEEQAQSLI DKTTAAFQQG KIPPTPFSAP PPAGAMIPPP PSLPGPPRPG MMPAPHMGGP PMMPMMGPPP PGMMPVGPAP GMRPPMGGHM PMMPGPPMMR PPARPMMVPT RPGMTRPDR Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
