Recombinant protein corresponding to Lys160-Arg399 of mouse TBX21, expressed in E. coli.
TBX21 expression correlates with IFNG expression in Th1 and natural killer (NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Retroviral gene transduction of TBX21 into polarized Th2 and Tc2 primary T cells redirected them into Th1 and Tc1 cells, respectively, as evidenced by the simultaneous induction of IFNG and repression of interleukin-4 (IL4) and interleukin-5 (IL5). The murine Tbx21 gene encodes a deduced 530aa protein containing a 189aa T-box DNA-binding domain that shows homology to murine Tbr1) and Xenopus eomesodermin (EOMES). The authors subsequently cloned human TBX21, which encodes a 535aa protein that shares 88% aa identity with murine Tbx21. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- K QGRRMFPFLS FTVAGLEPTS HYRMFVDVVL VDQHHWRYQS GKWVQCGKAE GSMPGNRLYV HPDSPNTGAH WMRQEVSFGK LKLTNNKGAS NNVTQMIVLQ SLHKYQPRLH IVEVNDGEPE AACSASNTHV FTFQETQFIA VTAYQNAEIT QLKIDNNPFA KGFRENFESM YASVDTSVPS PPGPNCQLLG GDPFSPLLSN QYPVPSRFYP DLPGQPKDMI SQPYWLGTPR EHSYEAEFR Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
