Recombinant protein corresponding to Thr585-Lys771 of chicken TFR, fused to two N-terminal Tags, His-Tag and S-tag expressed in E. coli.
Transferrin receptor is a carrier protein for transferrin. It is needed for the import of iron into the cell and is regulated in response to intracellular iron concentration. Low iron concentrations promote increased levels of transferrin receptor, to increase iron intake into the cell. Thus, transferrin receptor maintains cellular iron homeostasis. Expression of human TFR1, but not human TFR2, in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses, but not with those of Lassa or lymphocytic choriomeningitis viruses. An anti-TFR1 antibody efficiently inhibited the replication of Machupo, Guanarito, Junin, and Sabia viruses, but not that of Lassa virus. TFR1 is a cellular receptor for New World hemorrhagic fever arenaviruses. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- TKGDTL NLRKIDNLD ALLAAAAEVA GQAALRLTHD HELFLDIGRY SEELLAYQEE FLPYIKEVRE LGLTLDWLFF ARGDFQRAVT ALRRDIANSD GENRVIRRAL NDRMMKVEYD FLSPYLSPKD VPFRHIFFGK GPHTLRSLVE HLQLLKTNRS SVDLNLLREQ LALATWTIKG RPMPWEVISG K Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
