Tie2 (TEK Tyrosine Kinase, Endothelial, CD202b, CD202-B, TEK, VMCM1, Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2, Venous Malformations Multiple Cutaneous And Mucosal), Rabbit
Artikelnummer:
USB-365303
Hersteller Artikelnummer:
365303
Alternativnummer:
USB-365303-200
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Gly286-Ile46 of human Tie2, expressed in E. coli.
Tie-2 is a receptor tyrosine kinase that is expressed primarily on endothelial cells (ECs) and plays a critical role in vascular development. The Tie-2 gene encodes a protein of 1122 aa. The extracellular region has three distinct structural motifs including two immunoglobulin (Ig)-like loops separated by three EGF-like repeats, and three repeats with fibronectin type III homology located after the second Ig loop. The intracellular portion of Tie-2 contains two tyrosine kinase domains that, when phosphorylated, interact with a number of binding partners including Grb2, Grb7, Grb14, Shp2, the p85 subunit of phosphatidylinositol 3-kinase (PI3K), and Dok-R. Deletion of the last 16 aa of the intracellular C-terminus results in increased levels of autophosphorylation, suggesting that it may play an autoinhibitory role. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
