PICK1, CT (PRKCA-binding Protein, Protein Interacting with C Kinase 1, Protein Kinase C-alpha-binding Protein), Rabbit
Artikelnummer:
USB-366981
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| Artikelname: | PICK1, CT (PRKCA-binding Protein, Protein Interacting with C Kinase 1, Protein Kinase C-alpha-binding Protein), Rabbit |
| Artikelnummer: | USB-366981 |
| Hersteller Artikelnummer: | 366981 |
| Alternativnummer: | USB-366981-100 |
| Hersteller: | US Biological |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IHC, WB |
| Immunogen: | GST-tagged, recombinant protein corresponding to mouse PICK1 C-terminal fragment. |
| PRKCA-binding protein (UniProt Q62083, also known as Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein) is encoded by the Pick1 (also known as Prkcabp) gene (Gene ID 18693) in murine species. PICK1 is a scaffolding protein expressed in many tissues, where it regulates trafficking of multiple membrane proteins. The PDZ (PSD-95/Dlg/ZO-1/DHR (Dlg homologous region) domain (aa22-105) mediates PICK1 interaction with proteins containing PDZ-binding motifs, such as PKC and acid sensing ion channels (ASICs), while the lipid-binding AH (arfaptin homology)/BAR (Bin/amphiphysin/Rvs) domain (aa144-357) mediates PICK1 homo-dimerization and membrane localization. Pick1 gene-disruption results in downregulated ASICs current and calcium transients during process of ischemia-reperfusion and protects against primary murine cortical neurons against injury caused by OGD-Rep (oxygen and glucose deprivation followed by reperfusion). PICK1 is also shown to act as a negatively regulator against ASIC1 phosphorylation/activation by PKA in rat pulmonary arterial smooth muscle cells, where PICK1 facilitates calcineurin-mediated ASIC1 dephosphorylation. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:1000 detects PICK1 in human cerebellum, kidney, and pancreas tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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