The CD19 antigen is present on approximately 7% to 23% of human peripheral blood lymphocytes and on spleenocytes, and on human B lymphocytes at all stages of maturation but is lost on terminally differentiated plasma cells. CD19 does not react with resting or activated T lymphocytes, granulocytes, or monocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.