Beta-galactosidase, also called beta-gal or Beta-gal, is a hydrolase enzyme that catalyzes the hydrolysis of Beta-galactosides into monosaccharides. Substrates of different Beta-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. Lactase is often confused as an alternative name for Beta-galactosidase, but it is actually simply a sub-class of Beta-galactosidase. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:50,000 Western Blot: 1:2000-1:10,000 Immunofluorescence: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by Ion Exchange chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.01% sodium azide.
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