Protein A, Recombinant. Expressed in soy-based media, free of animal proteins. Source: Recombinant protein corresponding to Protein A, expressed in E. coli. Bioburden: No organisms detected. Endotoxin: 0.5EU/mg Protein Concentration (OD275): ~50mg/ml 10% SDS-PAGE (Coomassie Stain): Single major band 45kD1kD hIgG Binding: 95% Conductivity: <0.1mS/cm UV Spectrum: E270/250 >1.2 E0.1% at 270nm = 0.14-0.20 Triton Content, HPLC: <0.015% Isoelectric Focusing: Single band between 4.6 and 5.2 pI markers. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
214nm: 98%, 280nm: 95% (HPLC)
Formulierung:
Supplied as a liquid. Expressed in soy-based media, free of animal proteins.
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