CD27, as known as CD27 antigen, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycosylation on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. This protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Source: Recombinant protein corresponding to aa21-191 from human CD27, fused to hlgG-His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~46.4kD (413aa) 28-57kD (SDS-PAGE under reducing conditions) Amino Acid Sequence: ADPTPAPKSC PERHYWAQGK LCCQMCEPGT FLVKDCDQHR KAAQCDPCIP GVSFSPDHHT RPHCESCRHC NSGLLVRNCT ITANAECACR NGWQCRDKEC TECDPLPNPS LTARSSQALS PHPQPTHLPY VSEMLEARTA GHMQTLADFR QLPARTLSTH WPPQRSLCSS DFIRLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH Endotoxin: ~1EU/1ug (LAL) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
