Carboxylesterase 1/CES1, Recombinant, Mouse, aa19-565, His-Tag (Liver carboxylesterase 1, Carboxylesterase 1/CES1, CES1G, Ces-1, Ces1, Ses-1, Acyl-coenzyme A:cholesterol acyltransferase, Carboxylesterase 1G, ES-x)

Produktübersicht

• Artikelname: Carboxylesterase 1/CES1, Recombinant, Mouse, aa19-565, His-Tag (Liver carboxylesterase 1, Carboxylesterase 1/CES1, CES1G, Ces-1, Ces1, Ses-1, Acyl-coenzyme A:cholesterol acyltransferase, Carboxylesterase 1G, ES-x)
• Artikelnummer: USB-369033
• Hersteller Artikelnummer: 369033
• Alternativnummer: USB-369033-50,USB-369033-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

CES1G, also known as liver carboxylesterase 1, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. CES1G shares the serine hydrolase fold observed in other esterases. It is a rat and mouse specific protein that is expressed predominantly in liver, but also in kidney and lung. Recombinant protein corresponding to aa19-565 from mouse CES1G, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~61.9kD (556aa), ~50-70kD (SDS-PAGE under reducing conditions.) Specific Activity: >2000 pmol/min/ug Unit Definition: The amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol/minute at pH 7.5 at 37C Amino Acid Sequence: ADPHPSLPPV VHTVHGKVLG KYVTLEGFSQ PVAVFLGVPF AKPPLGSLRF APPEPAEPWS FVKHTTSYPP LCYQNPEAAL RLAELFTNQR KIIPHKFSED CLYLNIYTPA DLTQNSRLPV MVWIHGGGLV IDGASTYDGV PLAVHENVVV VVIQYRLGIW GFFSTEDEHS RGNWGHLDQV AALHWVQDNI ANFGGNPGSV TIFGESAGGE SVSVLVLSPL AKNLFHRAIA QSSVIFNPCL FGRAARPLAK KIAALAGCKT TTSAAMVHCL RQKTEDELLE VSLKMKFGTV DFLGDPRESY PFLPTVIDGV LLPKAPEEIL AEKSFNTVPY MVGINKHEFG WIIPMFLDFP LSERKLDQKT AASILWQAYP ILNISEKLIP AAIEKYLGGT EDPATMTDLF LDLIGDIMFG VPSVIVSRSH RDAGAPTYMY EYQYRPSFVS DDRPQELLGD HADELFSVWG APFLKEGASE EEINLSKMVM KFWANFARNG NPNGEGLPHW PEYDQKEGYL QIGVPAQAAH RLKDKEVDFW TELRAKETAE RSSHREHVEL HHHHH H Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 61.9
• NCBI: 067431
• UniProt: Q8VCC2
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL)
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.