NAPSA, also known as Napsin-A, belongs to the peptidase A1 family and may be involved in processing of pneumocyte surfactant precursors. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminal. Source: Recombinant protein corresponding to aa64-420 from human NAPSA, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~40.9kD (380aa) confirmed by MALDI-TOF AA Sequence: MGSSHHHHHH SSGLVPRGSH QANGTKFAIQ YGTGRVDGIL LNRDPEEPDG GELVLGGSDP GEYIILCSEI PKLPAVSFLL ARTRGADLGW GETAQAQFPG MGSKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS NLWVPSRRCH FFSVPCWLHH RFDPKASSSF SEDKLTIGGI KGASVIFGEA LWEPSLVFAF AHFDGILGLG FPILSVEGVR PPMDVLVEQG LLDKPVFSFY AHYIPPLTFV PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH AAIGGIPLLA GGVWFNLTAH DYVIQTTRNG VRLCLSGFQA LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
