Th, also known as tyrosine 3-monooxygenase, is a rate-limiting enzyme in catecholamine synthesis. It uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to DOPA. It regulates dopamine (DA) neurotransmission at the biosynthesis and reuptake steps. It plays an important role in the physiology of adrenergic neurons. It effects overexpression in lymphocytes on the differentiation and function of T helper cells. Source: Recombinant protein corresponding to aa1-498 from mouse Th, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~57.0kD (507aa) 50-70kD (SDS-PAGE under reducing conditions) Amino Acid Sequence: ADPMPTPSAS SPQPKGFRRA VSEQDTKQAE AVTSPRFIGR RQSLIEDARK EREAAAAAAA AAVASAEPGN PLEAVVFEER DGNAVLNLLF SLRGTKPSSL SRALKVFETF EAKIHHLETR PAQRPLAGSP HLEYFVRFEV PSGDLAALLS SVRRVSDDVR SAREDKVPWF PRKVSELDKC HHLVTKFDPD LDLDHPGFSD QAYRQRRKLI AEIAFQYKQG EPIPHVEYTK EEIATWKEVY ATLKGLYATH ACREHLEAFQ LLERYCGYRE DSIPQLEDVS HFLKERTGFQ LRPVAGLLSA RDFLASLAFR VFQCTQYIRH ASSPMHSPEP DCCHELLGHV PMLADRTFAQ FSQDIGLASL GASDEEIEKL STVYWFTVEF GLCKQNGELK AYGAGLLSSY GELLHSLSEE PEVRAFDPDT AAVQPYQDQT YQPVYFVSES FSDAKDKLRN YASRIQRPFS VKFDPYTLAI DVLDSPHTIR RSLEGVQDEL HTLTQALSAI SHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
