THY1, as known as Thy-1 membrane glycoprotein, is a cell membrane protein which contains 1lg-like V-type (immunoglobulin-like) domain. This protein is a member of the immunoglobulin superfamily of adhesion molecules with constitutive expression on fibroblast cells. It can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Also, this protein led to some of the initial biochemical description and characterization of a vertebrate glycophosphatidylinositol (GPI) anchor and also the first demonstration of tissue specific differential glycosylation. Source: Recombinant protein corresponding to aa20-130 from human THY1, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~13.3kD (117aa) 13.5-28kD (SDS-PAGE under reducing conditions) Amino Acid Sequence: QKVTSLTACL VDQSLRLDCR HENTSSSPIQ YEFSLTRETK KHVLFGTVGV PEHTYRSRTN FTSKYNMKVL YLSAFTSKDE GTYTCALHHS GHSPPISSQN VTVLRDKLVK CHHHHHH Endotoxin: ~1EU/1ug (LAL) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
