NHE3 (Sodium/Hydrogen Exchanger 3, SLC9A3, Solute Carrier Family 9 Member 3, Sodium-hydrogen Antiporter 3, Na(+)/H(+) Exchanger 3), Rabbit
Artikelnummer:
USB-369594
Hersteller Artikelnummer:
369594
Alternativnummer:
USB-369594-200
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IHC, IP, WB
An apical membrane Na+/H+ exchanger is involved in transepithelial, electroneutral Na+ absorption across renal and intestinal epithelia. The apical Na+/H+ exchange activity is pharmacologically distinct from the basolateral Na+/H+ exchange activity, notably in terms of response to kinase regulation and in addition by the diuretic amiloride. NHE1 (SLC9A1), the first of these exchangers to be cloned, localizes by immunocytochemical studies only to the basolateral membranes of polarized epithelial cells. Transfection of OKP cells with dominant-negative PYK2 or small interfering PYK2 duplex RNA blocked acid activation of NHE3, whereas neither had an effect on glucocorticoid activation of NHE3. Dominant-negative PYK2 also blocked acid activation of SRC kinase, which is required for acid regulation of NHE3. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.2-2ug/ml Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
