Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. Splits ManA-ManA and ManA-GulA bonds, but not GulA-ManA or GulA-GulA bonds. Also cleaves acetylated residues (PubMed:9683471). May serve to degrade mislocalized alginate that is trapped in the periplasmic space (By similarity). Source: Recombinant protein corresponding to aa24-374 from Azotobacter vinelandii AlgL, fused to 10xHis-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. Molecular Weight: ~59.0kD AA Sequence: AEALVPP KGYYAPVDIR KGEAPACPVV PEPFTGELVF RSKYEGSDAA RSTLNEEAEK AFRTKTAPIT QIERGVSRMV MRYMEKGRAG DLECTLAWLD AWAEDGALLT TEYNHTGKSM RKWALGSLAG AYLRLKFSSS QPLAAYPEQA RRIESWFAKV GDQVIKDWSD LPLKRINNHS YWAAWAVMAA GVATNRRPLF DWAVEQFHIA AGQVDSNGFL PNELKRRQRA LAYHNYSLPP LMMVAAFALA NGVDLRGDND GALGRLAGNV LAGVEKPEPF AERAGDEDQD MEDLETDAKF SWLEPYCALY SCSPALRERK AEMGPFKNFR LGGDVTRIFD PAEKSPRSTV GKRD Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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