Blm, Recombinant, Mouse, aa684-859, His-Tag, Myc-Tag (Bloom Syndrome Protein Homolog)

Produktübersicht

• Artikelname: Blm, Recombinant, Mouse, aa684-859, His-Tag, Myc-Tag (Bloom Syndrome Protein Homolog)
• Artikelnummer: USB-372456
• Hersteller Artikelnummer: 372456
• Alternativnummer: USB-372456-20, USB-372456-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3-5 direction. Involved in 5-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5-ssDNA. Negatively regulates sister chromatid exchange (SCE). Source: Partial recombinant protein corresponding to aa684-859 from mouse Blm, fused to 10xHis-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. Molecular Weight: ~24.7kD Amino Acid Sequence: INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 24.7
• UniProt: O88700
• Reinheit: 90% (SDS-PAGE)
• Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.