May act as transcriptional regulator. Induces apoptosis in infected cells. Element of infectious replication cycle. VP1 and VP2 are detected 12 hours post infection, while VP3 only after 24 hours. Belongs to the gyrovirus apoptin family. Recombinant full length protein corresponding to aa1-121 from chicken anemia virus Apoptin, fused to GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~40.3kD Amino Acid Sequence: MNALQEDTPPGPSTVFRPPTSSRPLETPHCREIRIGIAGITITLSLCGCANARAPTLRSATADNSESTGFKNVPDLRTDQPKPPSKKRSCDPSEYRVSELKESLITTTPSRPRTAKRRIRL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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