GlaB1, Recombinant, Bacteroides Vulgatus, aa19-592, His-Tag (Alpha-1,3-galactosidase B)
Artikelnummer:
USB-373442
Hersteller Artikelnummer:
373442
Alternativnummer:
USB-373442-20, USB-373442-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures. Recombinant protein corresponding to aa19-592 from bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154) GlaB1, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~68.6kD Amino Acid Sequence: SAQKVYEISAFGLKANSSKNASPVLQKALAKIKAEYKEGEKVILRFPEGRYEFHEKGAAVREYYISNHDQTNPKKVGIALEDMKNLTLDGQGSEFVFHGRMLPVSLLRSENCLLKNFSIDFENPHIAQVKIVENDPQDGIVFEPAPWVDYRIAKDSIFEAYGEGWTMRHSWGIAFDGDTKHLVYNTSDIGCPTKGASEVAPRRIHAPGWKDARLVPGTVVAMRGWGRPTPGIFLSHDVNTTIENVKVHYAEGMGLLAQLCENITLEKFGVCLKGDADPRYFTTQADATHFSGCKGKIVSCNGLYEGMMDDAINVHGTYLKVVKRVDDRTLVGRYMHGQSWGFEWGCPGDEVQFIRSNTMELVGKQNKIISIRPYDKEQTEGAREFLITFQEPVDQVINEQSGFGIENLTWTPEVLFSGNVIRNNRARGSLFSTPRKTIVENNLFDHTSGAAILLCGDCNGWFETGACRHVIIRKNRFVNALTNLFQFTNAVISIYPEIPDLKGQQQYFHGGPEGGIVIEDNEFETFDAPILYAKSVDGLVFRNNTIKLNTEYKPFHPNRNRFWLERVTNVTIAE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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