HPH, Recombinant, E. coli, aa1-341, His-Tag (Hygromycin-B 4-O-Kinase)

Produktübersicht

• Artikelname: HPH, Recombinant, E. coli, aa1-341, His-Tag (Hygromycin-B 4-O-Kinase)
• Artikelnummer: USB-373678
• Hersteller Artikelnummer: 373678
• Alternativnummer: USB-373678-20, USB-373678-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin. Source: Recombinant protein corresponding to aa1-341 from E. coli hph, fused to His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~40.0kD Amino Acid Sequence: MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 40
• UniProt: P00557
• Reinheit: 90% (SDS-PAGE)
• Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.