RecF, Recombinant, E. coli, aa2-357, His-SUMO-Tag (DNA Replication and Repair Protein RecF)

Produktübersicht

• Artikelname: RecF, Recombinant, E. coli, aa2-357, His-SUMO-Tag (DNA Replication and Repair Protein RecF)
• Artikelnummer: USB-375018
• Hersteller Artikelnummer: 375018
• Alternativnummer: USB-375018-20,USB-375018-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

The RecF protein is involved in DNA metabolism, it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. Recombinant protein corresponding to aa2-357 from E. coli recF, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~56.4kD Amino Acid Sequence: SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 56.4
• UniProt: P0A7H0
• Reinheit: ~90% (SDS-PAGE)
• Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.