RibA, Recombinant, E. coli, aa1-196, His-Tag (GTP Cyclohydrolase-2)
Artikelnummer:
USB-375059
Hersteller Artikelnummer:
375059
Alternativnummer:
USB-375059-20,USB-375059-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5-phosphate (DARP), formate and pyrophosphate. Source: Recombinant protein corresponding to aa1-196 from E. coli GTP Cyclohydrolase-2, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~25.9kD Amino Acid Sequence: MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHTPVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKIRAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTEAGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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