SPT15, Recombinant, S. cerevisiae, aa2-240, His-Tag (TATA-Box-Binding Protein)

Produktübersicht

• Artikelname: SPT15, Recombinant, S. cerevisiae, aa2-240, His-Tag (TATA-Box-Binding Protein)
• Artikelnummer: USB-375399
• Hersteller Artikelnummer: 375399
• Alternativnummer: USB-375399-20,USB-375399-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Source: Recombinant protein corresponding to aa2-240 from S. cerevisiae SPT15, fused to His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~28.9kD Amino Acid Sequence: ADEERLKEFKEANKIVFDPNTRQVWENQNRDGTKPATTFQSEEDIKRAAPESEKDTSATSGIVPTLQNIVATVTLGCRLDLKTVALHARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVVTGAKSEDDSKLASRKYARIIQKIGFAAKFTDFKIQNIVGSCDVKFPIRLEGLAFSHGTFSSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAKQREEIYQAFEAIYPVLSEFRKM Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 28.9
• UniProt: P13393
• Reinheit: 90% (SDS-PAGE)
• Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.