PRMT8, Chemiluminescent BioAssay(TM) Kit (Protein Arginine N-methyltransferase 8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4)

Produktübersicht

• Artikelname: PRMT8, Chemiluminescent BioAssay(TM) Kit (Protein Arginine N-methyltransferase 8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4)
• Artikelnummer: USB-377020
• Hersteller Artikelnummer: 377020
• Alternativnummer: USB-377020-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Intended Use: Suitable for studying enzyme kinetics and HTS applications. Test Principle: The PRMT8 Chemiluminescent BioAssay(TM) Kit is designed to measure PRMT8 activity for screening and profiling applications. The PRMT8 Chemiluminescent BioAssay(TM) Kit comes in a convenient format with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine 3 (R3) residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified PRMT8 enzyme for 100 enzyme reactions. The key to the PRMT8 Chemiluminescent BioAssay(TM) Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Kit Components: PRMT8 His-FLAG-tags, 1x10ug 20uM S-adenosylmethionine*, 1x250ul Primary antibody 4, 1x100ul Secondary HRP-labeled antibody 2, 1x10ul 4x HMT assay buffer 2, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent substrate A (transparent bottle), 1x6ml HRP chemiluminescent substrate B (brown bottle), 1x6ml 96-well plate precoated with histone substrate, 1x plate * Decreasing the S-adenosylmethionine concentration will make the assay more sensitive to test inhibitors. Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.