Beta-lactaminase, Recombinant, E. coli, aa20-377, His-Tag (AmpC, AmpA, Cephalosporinase)
Artikelnummer:
USB-377716
Hersteller Artikelnummer:
377716
Alternativnummer:
USB-377716-20, USB-377716-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecules antibacterial properties. Source: Recombinant protein corresponding to aa20-377 from beta-lactamase protein, fused to His-Tag at N-terminal, expressed in E. coli. Accession: NP_418574. Molecular Weight: ~41.8kD (379aa), confirmed by MALDI-TOF Biological Activity: Specific activity is >700units/mg, in which One unit will hydrolyze 1umole of Nitrocefin/minute at pH 7.0 at 37C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
