Ppib, also known as peptidyl-prolyl cis-trans isomerase B, is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A -mediated immunosuppression. Source: Recombinant protein corresponding to aa24-188 from mouse Ppib, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~22.7kD (207aa), confirmed by MALDI-TOF Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Specific activity is >1000nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA/minute at 37C in Tris-HCl pH 8.0 using chymotrypsin. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNDKKKG PKVTVKVYFD LQIGDESVGR VVFGLFGKTV PKTVDNFVAL ATGEKGFGYK NSKFHRVIKD FMIQGGDFTR GDGTGGKSIY GERFPDENFK LKHYGPGWVS MANAGKDTNG SQFFITTVKT SWLDGKHVVF GKVLEGMDVV RKVESTKTDS RDKPLKDVII VDSGKIEVEK PFAIAKE Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
