Ppif, Recombinant, Rat, aa30-206, His-Tag (Peptidyl-prolyl Cis-trans Isomerase F, Mitochondrial, CyP-D, CypD, PPIase)
Artikelnummer:
USB-377936
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| Artikelname: | Ppif, Recombinant, Rat, aa30-206, His-Tag (Peptidyl-prolyl Cis-trans Isomerase F, Mitochondrial, CyP-D, CypD, PPIase) |
| Artikelnummer: | USB-377936 |
| Hersteller Artikelnummer: | 377936 |
| Alternativnummer: | USB-377936-20,USB-377936-100 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Source: Recombinant protein corresponding to aa24-188 from rat Ppif, fused to His-Tag, expressed in E. coli. Molecular Weight: ~21.2kD (200aa), confirmed by MALDI-TOF Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Specific activity is >1300nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAFP-PNA/minute at 37C in Tris-HCl pH 8.0 using chymotrypsin. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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