RAET1E, also known as NKG2D ligand 4 isoform 1, is a member of the RAET1/ULBP family of cell surface protein that function as ligands for NKG2D. This protein is abnormally expressed on most colon cancer and some other tumor cell lines and virus infected peripheral blood cells. It binds and co-stimulates NKG2D expressing effector cells including NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells, activating cytolytic activity and/or cytokine production. Also, it functions as a stress-induced ligand for NKG2D receptor. Source: Recombinant protein corresponding to aa24-188 from human RAET1E, fused to hIgG-His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~49.6kD (437aa), 57-70kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADPHSLCFNF TIKSLSRPGQ PWCEAQVFLN KNLFLQYNSD NNMVKPLGLL GKKVYATSTW GELTQTLGEV GRDLRMLLCD IKPQIKTSDP STLQVEMFCQ REAERCTGAS WQFATNGEKS LLFDAMNMTW TVINHEASKI KETWKKDRGL EKYFRKLSKG DCDHWLREFL GHWEAMPEPT VSPVNASDIH WSSSSLPDVE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
