Serpina9, also known as serpin A9, is a member of the Serpin superfamily of serine protease inhibitors. Serpins are the most broadly distributed superfamily of protease inhibitors that use a conformational change to inhibit target enzymes. They are known to inhibit serine proteases and some are known to inhibit caspases and papain-like cysteine proteases. Serpins are conformational labile and many of the disease-linked mutations of serpins result in misfolding or in pathogenic, inactive polymers. This protein shows inhibition towards trypsin, thrombin, and plasmin and binds DNA and heparin. Source: Recombinant protein corresponding to aa26-418 from mouse Serpina9, fused to His-Tag at C- terminal, expressed in insect cell. Molecular Weight: ~45.2kD (401aa), 50-70kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: NPYNQESSHL PSMKKNPASQ VSPSNTRFSF LLYQRLAQEN PGQNILFSPV SISTSLAMLS LGARSATKTQ ILRTLGFNFT WVSEPTIHMG FEYLVRSLNK CHQGRELRMG SVLFIRKELQ LQATFLDRVK KLYGAKVFSE DFSNAATAQA QINSYVEKET KGKVVDVIQD LDSQTAMVLV NHIFFKANWT QPFSTANTNK SFPFLLSKGT TVHVPMMHQT ESFAFGVDKE LGCSILQMDY RGDAVAFFVL PGKGKMRQLE KSLSARRLRK WSRSLQKRWI KVFIPKFSIS ASYNLETILP KMGIRDAFNS NADFSGITKT HFLQVSKAAH KAVLDVSEEG TEAAAATTTK LIVRSRDTPS SIIAFKEPFL ILLLDKNTES VLFLGKVENP RKMLEHHHHH H Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
