VCAM1, also known as vascular cell adhesion protein 1 isoform, a member of the immunoglobulin superfamily. It is a cell surface protein expressed by activated endothelial cells and certain leukocytes. VCAM1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection and asthmatic responses. During the inflammatory adhesion mechanism, activated integrins that rolling leukocytes and attach them firmly to the vascular endothelium. Source: Recombinant protein corresponding to aa25-698 from human VCAM1, fused to His-Tag at C-terminal, was expressed in insect cell. Molecular Weight: ~75.6kD (686aa), 70-100kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 Human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates 10ug/ml. This effect is more to 70%. AA Sequence: ADPEFFKIET TPESRYLAQI GDSVSLTCST TGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDP EIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDI GKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWS KKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGD SVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVE LYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMT FIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGF PAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFP SESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLR SLTLDVQGRE NNKDYFSPEH HHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
