Troponin T Type 2, Cardiac (cTnT/TNNT2) BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer:
USB-382540
Hersteller Artikelnummer:
382540
Alternativnummer:
USB-382540-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
This Troponin T Type 2, Cardiac (cTnT/TNNT2) BioAssay(TM) ELISA Kit is a sandwich ELISA for in vitro quantitative measurement of cTnT/TNNT2 in mouse serum, plasma and other biological fluids. Detection Detection Range: 15.63-1000pg/ml Sensitivity: 9.38pg/ml Precision: Intra-Assay %CV: <10% Inter-Assay %CV: <10% Specificity: Recognizes mouse cTnT/TNNT. No significant crossreactivity or interference between mouse cTnT/TNNT and analogues was observed. Test Principle: The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to mouse cTnT/TNNT2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for mouse cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain mouse cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of mouse cTnT/TNNT2. The concentration of mouse cTnT/TNNT2 in samples can be calculated by comparing the OD of the samples to the standard curve. Kit Components: *382540A: Microtiter Strips, 1x96 wells (8x12wells), Pre-coated, ready to use *382540B: Standard, 2x1vial 382540C: Standard Diluent, 1x20ml *382540D: Biotin Detection, 100X, 1x120ul *382540E: HRP Conjugate, 100X, 1x120ul 382540F: Biotin Detection Diluent, 1x14ml 382540G: HRP Diluent, 1x14ml 382540H: Substrate, 1x10ml 382540K: Stop Solution, 1x10ml 382540L: Wash Buffer, 25X, 1x30ml Precaution: The Stop Solution (382540K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *382540A, *382540B, *382540D and *382540E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 90 minutes at 37C. 3. Aspirate and add 100ul prepared Biotin Working Solution. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul preparedHRP Detection Working Solution. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate. Incubate 15 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
