Troponin I Type 3, Cardiac (TNNI3/cTn-I) BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer:
USB-384692
Hersteller Artikelnummer:
384692
Alternativnummer:
USB-384692-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
The Troponin I Type 3, Cardiac (TNNI3/cTn-I) BioAssay(TM) ELISA Kit is a sandwich ELISA for in vitro quantitative measurement of TNNI3/cTn-I in mouse serum, plasma and other biological fluids. Detection Range: 7.81-500pg/ml Sensitivity: 4.69pg/ml Precision: Intra-Assay %CV: <10% Inter-Assay %CV: <10% Specificity: Recognizes mouse TNNI3/cTn-I. No significant crossreactivity or interference between mouse TNNI3/cTn-I and analogues was observed. Test Principle: This BioAssay(TM) ELISA kit uses Sandwich ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to mouse TNNI3/cTn-I. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for mouse TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain mouse TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm 2nm. The OD value is proportional to the concentration of mouse TNNI3/cTn-I. The concentration of mouse TNNI3/cTn-I in samples can be calculated by comparing the OD of the samples to the standard curve. Kit Components: *384692A: Microtiter Strips, 1x96 wells (8x12wells), Pre-coated, ready to use *384692B: Standard, 2x1vial 384692C: Standard Diluent, 1x20ml *384692D: Biotin Detection, 100X, 1x120ul *384692E: HRP Conjugate, 100X, 1x120ul 384692F: Biotin Detection Diluent, 1x14ml 384692G: HRP Diluent, 1x14ml 384692H: Substrate, 1x10ml 384692K: Stop Solution, 1x10ml 384692L: Wash Buffer, 25X, 1x30ml Precaution: The Stop Solution (384692K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *384692A, *384692B, *384692D and *384692E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 90 minutes at 37C. 3. Aspirate and add 100ul prepared Biotin Working Solution. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared HRP Detection Working Solution. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate. Incubate 15 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
