Trastuzumab (Herceptin) BioAssay(TM) ELISA Kit

Produktübersicht

• Artikelname: Trastuzumab (Herceptin) BioAssay(TM) ELISA Kit
• Artikelnummer: USB-384868
• Hersteller Artikelnummer: 384868
• Alternativnummer: USB-384868-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Trastuzumab (Herceptin) is a humanized monoclonal antibody used in the treatment of HER2-positive cancers. Trastuzumab binds to domain IV of the extracellular domain of the HER2/neu receptor. Cells treated with Trastuzumab undergo cell cycle arrest during the G1 phase. It has been suggested that trastuzumab does not alter HER-2 expression, but decreases activation of AKT. The Trastuzumab (Herceptin) BioAssay(TM) ELISA Kit is designed to measure trastuzumab with high specificity and enhanced sensitivity in serum, EDTA-plasma and heparin-plasma. The assay design utilizes a pair of antibodies that allows the detection of the whole trastuzumab molecule in biological matrices. Principle of the Assay: This assay employs the sandwich enzyme immunoassay technique. Anti-Trastuzumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Trastuzumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Trastuzumab present in test samples. Sensitivity: 1.25ng/ml Recovery: 1000ng/ml of Trastuzumab was spiked in 10 lots of human serum. Recovery ranges are from 91-117% with an average recovery of 106%. Specificity: hIgG1, Rituximab, and Infliximab prepared at 250ng/ml were assayed and exhibited no cross-reactivity or interference. Precision: Intra-assay CV: <10% Inter-assay CV: <10% Kit Components: 384868A: Microtiter Strips, 1x96 wells 384868B: Calibrator, 1000ug/ml, 1x12ul 384868C: Calibrator Diluent, 1x1.8ml 384868D: Wash Buffer, 10X, 1x50ml 384868E: Assay Buffer, 1x50ml 384868F: Detection Reagent, 1000X, 1x17ul 384868G: TMB, 1x12ml 384868H: Stop Solution, 1x12ml Storage and Stability: Store all components at -20C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.