Heat Shock Protein 70 (HSP70) BioAssay(TM) ELISA Kit (High-Sensitivity)

Produktübersicht

• Artikelname: Heat Shock Protein 70 (HSP70) BioAssay(TM) ELISA Kit (High-Sensitivity)
• Artikelnummer: USB-385065
• Hersteller Artikelnummer: 385065
• Alternativnummer: USB-385065-96
• Hersteller: US Biological
• Kategorie: Kits/Assays
• Applikation: ELISA

Produktbeschreibung

HSP70 genes encode abundant heat-inducible 70kD HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Sample Type: Cell lysates, Serum, Tissue Intended Use: This ELISA BioAssay(TM) kit is used to quantitate HSP70 concentration in samples. Sensitivity: 0.02ng/ml Range: 0.55-35ng/ml Specificity: Recognizes canine, human, monkey, mouse and rat HSP70. Test Principle: This ELISA BioAssay(TM) Kit is for the detection of Human and cross-reactive sample types of Hsp70 in plasma, serum and cell lysates. Each BioAssay(TM) kit contains sufficient components to quantitate the Hsp70 concentration in up to 40 samples, tested in duplicate. Kit Components: ELISA Plate, 1x96wells Standard, 2xvials Standard and Sample Diluent, 1x50ml 10X Wash Buffer Concentrate, 1x100ml Biotinylated Antibody Concentrate, 1x150ul Biotinylated Antibody Diluent, 1x13ml Streptavidin: HRP Concentrate, 1x50ul Streptavidin: HRP Diluent, 1x13ml TMB Substrate, 1x13ml Stop Solution, 1x13ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.