Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Rabbit Cadherin-2/N-Cadherin. Sensitivity: <20pg/ml Range: 0.78ng/ml-50ng/ml Specificity: Recognizes natural and recombinant Rabbit Cadherin-2/N-Cadherin. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Rabbit Cadherin-2/N-Cadherin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit Cadherin-2/N-Cadherin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit Cadherin-2/N-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit Cadherin-2/N-Cadherin amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Rabbit CDH2 antibody, 1x plate Lyophilized recombinant Rabbit CDH2 standard, 2x50ng/tube Biotinylated anti-Rabbit CDH2 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
