Tyrosine-protein kinase receptor TYRO3 is an enzyme that in humans is encoded by the TYRO3 gene. The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Mouse DTK/TYRO3. Sensitivity: <10pg/ml Range: 125pg/ml-8000pg/ml Specificity: Recognizes natural and recombinant Mouse DTK/TYRO3. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Mouse DTK/TYRO3 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from rat specific for DTK/TYRO3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A31-S418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DTK/TYRO3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse DTK/TYRO3 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Mouse Tyro3 antibody, 1x plate Lyophilized recombinant Mouse Tyro3 standard, 2x10ng/tube Biotinylated anti-Mouse Tyro3 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
