FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Rat FABP4/A Fabp. Sensitivity: <15pg/ml Range: 156pg/ml-10,000pg/ml Specificity: Recognizes natural and recombinant Rat FABP4/A Fabp. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Rat FABP4/A Fabp BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for FABP4/A Fabp has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: M1-A132) and test samples are added to the wells, a biotinylated detection specific for FABP4/A Fabp is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat FABP4/A Fabp amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Rat Fabp4 antibody, 1x plate Lyophilized recombinant Rat Fabp4 standard, 2x10ng/tube Biotinylated anti-Rat Fabp4 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
