Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand- dependent manner. Sample Type: Cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human IL-23R. Sensitivity: <10pg/ml Range: 156pg/ml-10000pg/ml Specificity: Recognizes natural and recombinant Human IL-23R. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human IL-23R BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A a monoclonal antibody from mouse specific for IL-23R has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: I25-I354) and test samples are added to the wells, a biotinylated detection a biotinylated polyclonal antibody from goat specific for IL-23R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-23R amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human IL23R antibody, 1x plate Lyophilized recombinant Human IL23R standard, 2x10ng/tube Biotinylated anti-Human IL23R antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
