Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin. Sample Type: Cell culture supernates, serum. Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Mouse Marapsin/Pancresin. Sensitivity: <10pg/ml Range: 125pg/ml-8000pg/ml Specificity: Recognizes natural and recombinant Mouse Marapsin/Pancresin. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Mouse Marapsin/Pancresin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for Marapsin/Pancresin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: M38-V328) and test samples are added to the wells, a biotinylated detection specific for Marapsin/Pancresin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Marapsin/Pancresin amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Mouse Prss27 antibody, 1x plate Lyophilized recombinant Mouse Prss27 standard, 2x10ng/tube Biotinylated anti-Mouse Prss27 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
