Matrix metallopeptidase 9 (MMP-9) is also known as 92kD type IV collagenase, 92kD gelatinase or gelatinase B (GELB). The 72- and 92kD type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. Sample Type: Cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Rat MMP-9. Sensitivity: <15pg/ml Range: 312pg/ml-20,000pg/ml Specificity: Recognizes natural and recombinant Rat MMP-9. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Rat MMP-9 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for MMP-9 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L35-P708) and test samples are added to the wells, a biotinylated detection specific for MMP-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat MMP-9 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Rat Mmp9 antibody, 1x plate Lyophilized recombinant Rat Mmp9 standard, 2x20ng/tube Biotinylated anti-Rat Mmp9 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
